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Bcl-2 Family Interacting Proteins / Related Products
You are here: Product Lines > Cell Death / Apoptosis / Autophagy > Mitochondrial Pathway > Bcl-2 Family Members / Related Products > Bcl-2 Family Interacting Proteins / Related Products
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ALX-158-011 Revised 12-Sep-05
Humanin (human)
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PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-158-011-MC05   0.5 mg 210.00 USD Add To Cart
ALX-158-011-M001   1 mg 350.00 USD Add To Cart
Product Specification
SEQUENCE: H-Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala-OH
FORMULA: C119H204N34O32S2
MW: 2687.3
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO or 5% acetic acid.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
Product Description
Humanin was originally identified as an anti-apoptotic peptide of 24 aa encoded in a cDNA that rescued neuronal cells from apoptosis induced by presenilin mutants associated with familial Alzheimer's disease and by amyloid-β protein [1]. Mutagenesis studies showed that the cysteine at position 8 of the humanin peptide is critical for its anti-apoptotic function [1-3]. It has now been shown that Bax (Bcl-2 associated X protein), an apoptosis-inducing protein, interacts with humanin [4]. Humanin prevents the translocation of Bax from the cytosol to mitochondria. Humanin peptides also block Bax association with isolated mitochondria, and suppress cytochrome c release in vitro.
Product Specific Literature References
[1] A rescue factor abolishing neuronal cell death by a wide spectrum of familial Alzheimer's disease genes and Abeta: Y. Hashimoto, et al.; PNAS 98, 6336 (2001) Abstract; Full Text
[2] Detailed characterization of neuroprotection by a rescue factor humanin against various Alzheimer's disease-relevant insults: Y. Hashimoto, et al.; J. Neurosci. 21, 9235 (2001) Abstract; Full Text
[3] Mechanisms of neuroprotection by a novel rescue factor humanin from Swedish mutant amyloid precursor protein: Y. Hashimoto, et al.; BBRC 283, 460 (2001) Abstract
[4] Humanin peptide suppresses apoptosis by interfering with Bax activation: B. Guo, et al.; Nature 423, 456 (2003) Abstract
Further Categories Containing This Product:
PeptidesBcl-2 Family Interacting Proteins / Related Products
 
 
ALX-803-323 Revised 27-May-08 New product
Monoclonal Antibody to BAG-3 (AC-1)
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SYNONYMS anti-Bcl-2-binding Athanogene-3 MAb (AC-1)
anti-Docking Protein CAIR-1 MAb (AC-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-803-323-C100   100 µg 280.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: AC-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 18-33 (D18RDPLPPGWEIKIDPQ33) of N-terminal human BAG-3 (Bcl-2-binding athanogene 3).
SPECIFICITY: Recognizes human, mouse and rat BAG-3.
APPLICATION: ELISA
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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Product Specific Literature References
Bag3 gene expression is regulated by heat shock factor 1: S. Franceschelli, et al.; J. Cell Physiol. 215, 575 (2008) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-3 (CAIR-1; Bis) forms a complex with HSC70 and assists in polypeptide folding. In addition, this complex can mediate protein delivery to proteasome and is able to downregulate apoptosis by interfering with cytochrome c release, apoptosome assembly and other events. In human cancer cell lines, BAG-3 expression sustains cell survival and underlies resistance to therapy through downmodulation of apoptosis.
General Literature References
BAG3 protein controls B-chronic lymphocytic leukaemia cell apoptosis: M.F. Romano, et al.; Cell Death Differ. 10, 383 (2003) Abstract; Full Text
Apoptosis inhibition in cancer cells: a novel molecular pathway that involves BAG3 protein: A. Rosati, et al.; Int. J. Biochem. Cell Biol. 39, 1337 (2007) Abstract
 
 
ALX-804-367 Revised 04-Nov-08
Monoclonal Antibody to Bif-1 (human) (30A882.1.1)
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SYNONYMS anti-Bax Interacting Factor-1 (human) MAb (30A882.1.1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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ALX-804-367-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 30A882.1.1
ISOTYPE: Mouse IgG
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human Bif-1 (Bax interacting factor-1).
SPECIFICITY: Recognizes human Bif-1.
APPLICATION: Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY:

Stable for 6 months when stored at +4°C.

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Product Specific Literature References
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax: S.M. Cuddeback, et al.; J. Biol. Chem. 276, 20559 (2001) Abstract; Full Text
Endophilin B1 is required for the maintenance of mitochondrial morphology: M. Karbowski, et al.; J. Cell Biol. 166, 1027 (2004) Abstract
General Literature References
Bif-1 interacts with Beclin 1 through UVRAG and regulates autophagy and tumorigenesis: Y. Takahashi, et al.; Nat. Cell Biol. 9, 1142 (2007) Abstract
Further Categories Containing This Product:
Monoclonal AntibodiesAutophagy Other Products
 
 
ALX-210-011 Revised 22-Apr-08
Polyclonal Antibody to BAG-1 (Bur 1680)
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SYNONYMS anti-Bcl-2 Associated Athanogene-1 PAb (Bur 1680)
anti-BAG Family Molecular Chaperone Regulator 1 (Bur 1680) PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-011-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse BAG-1 (Bcl-2 associated athanogene-1) (aa 8-219).
SPECIFICITY: Recognizes mouse and rat BAG-1.
APPLICATION: Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (1:5'000))
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones 8, 225 (2003) Abstract; Full Text
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005) Abstract
 
 
ALX-210-009 Revised 22-Apr-08
Polyclonal Antibody to BAG-1 (mouse) (CT) (Bur 1702)
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SYNONYMS anti-Bcl-2 Associated Athanogene-1 (mouse) (CT) PAb (Bur 1702)
anti-BAG Family Molecular Chaperone Regulator 1 (mouse) (CT) (Bur 1702) PAb
anti-RAP46 (mouse) (CT) (Bur 1702) PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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ALX-210-009-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 204-219 (C204QETERLQSTNLALAE219) of mouse BAG-1 (Bcl-2 associated athanogene-1).
SPECIFICITY: Recognizes mouse BAG-1.
APPLICATION: Immunohistochemistry (paraffin sections (1:2'500))
Immunoprecipitation (transfected cells)
Western Blot (1:500-1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Cloning and functional analysis of BAG-1: a novel Bcl-2-binding protein with anti-cell death activity: S. Takayama, et al.; Cell 80, 279 (1995) Abstract
Interleukin-2 (IL-2) upregulates BAG-1 gene expression through serine-rich region within IL-2 receptor beta c chain: M. Adachi, et al.; Blood 88, 4118 (1996) Abstract; Full Text
Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1: H.G. Wang, et al.; PNAS 93, 7063 (1996) Abstract; Full Text
Role of Bag-1 in the survival and proliferation of the cytokine-dependent lymphocyte lines, Ba/F3 and Nb2: C.V. Clevenger, et al.; Mol. Endocrinol. 11, 608 (1997) Abstract; Full Text
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones 8, 225 (2003) Abstract; Full Text
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005) Abstract
 
 
ALX-210-010 Revised 22-Apr-08
Polyclonal Antibody to BAG-1 (NT) (Bur 1735)
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SYNONYMS anti-Bcl-2 Associated Athanogene-1 (NT) PAb (Bur 1735)
anti-BAG-family Molecular Chaperone Regulator 1 (NT) (Bur 1735) PAb
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-010-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 26-46 (N26ERYDLLVTPQQGNSEPVVQD46) of mouse BAG-1 (Bcl-2 associated athanogene-1).
SPECIFICITY: Recognizes mouse and rat BAG-1.
APPLICATION: Immunocytochemistry (1:1'000)
Immunohistochemistry (paraffin sections (1:3'500))
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Bag1 is a regulator and marker of neuronal differentiation: P. Kermer, et al.; Cell Death Differ. 9, 405 (2002) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-1 functions as a nucleotide exchange factor of mammalian cytosolic HSC70, thereby triggering substrate unloading from the chaperone. In addition BAG-1 associates with the proteasome, playing a role in coordinating chaperone and degradation pathways. Aberrant BAG-1 expression has been observed in certain human cancers.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions: S. Alberti, et al.; Cell Stress Chaperones 8, 225 (2003) Abstract; Full Text
BAG-1: a multi-functional pro-survival molecule: P.A. Townsend, et al.; Int. J. Biochem. Cell. Biol. 37, 251 (2005) Abstract
 
 
ALX-210-538 Revised 22-Apr-08
Polyclonal Antibody to BAG-3 (TOS-2)
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SYNONYMS anti-Bcl-2-binding Athanogene-3 PAb (TOS-2)
anti-Docking Protein CAIR-1 PAb (TOS-2)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
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ALX-210-538-R050   50 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human BAG-3 (Bcl-2-binding athanogene-3).
SPECIFICITY: Recognizes human, mouse and rat BAG-3.
APPLICATION: Immunocytochemistry
Immunohistochemistry (frozen sections; paraffin sections (1:100-1:200))
Immunoprecipitation
Western Blot (1:1’000-1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
Product Specific Literature References
The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL): G. Chiappetta, et al.; J. Clin. Endocrinol. Metab. 92, 1159 (2007) Abstract; Full Text
Evidence for BAG3 modulation of HIV-1 gene transcription: A. Rosati, et al.; J. Cell. Physiol. 210, 676 (2007) Abstract
General Information
BAG (Bcl-2-associated athanogene) family proteins contain a BAG domain and interact with a variety of partners including heat shock proteins, steroid hormone receptors, Bcl-2 and Raf-1. BAG-1 and BAG-3 participate in a wide variety of cellular processes including signalling, transcription, stress response, and apoptosis. BAG-3 (CAIR-1; Bis) forms a complex with HSC70 and assists in polypeptide folding. In addition, this complex can mediate protein delivery to proteasome and is able to downregulate apoptosis by interfering with cytochrome c release, apoptosome assembly and other events. In human cancer cell lines, BAG-3 expression sustains cell survival and underlies resistance to therapy through downmodulation of apoptosis.
General Literature References
BAG3 protein controls B-chronic lymphocytic leukaemia cell apoptosis: M.F. Romano, et al.; Cell Death Differ. 10, 383 (2003) Abstract; Full Text
Apoptosis inhibition in cancer cells: a novel molecular pathway that involves BAG3 protein: A. Rosati, et al.; Int. J. Biochem. Cell Biol. 39, 1337 (2007), (Review) Abstract
 
 
ALX-210-016 Revised 02-Feb-07
Polyclonal Antibody to BAR (human) (AR-48)
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SYNONYMS anti-Bifunctional Apoptosis  Regulator (human) PAb (AR-48)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bcl-2 Family Interacting Proteins / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-016-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains PBS and 0.05% sodium azide.
IMMUNOGEN: Recombinant human BAR (bifunctional apoptosis regulator) (aa 1-139).
SPECIFICITY: Recognizes human BAR.
APPLICATION: Immunocytochemistry (1:1'000)
Immunohistochemistry (paraffin sections (1:3'000))
Western Blot (1:3'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
BAR: An apoptosis regulator at the intersection of caspases and Bcl-2 family proteins: H. Zhang, et al.; PNAS 97, 2597 (2000) Abstract; Full Text
Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways: W. Roth, et al.; Cell Death Differ. 10, 1178 (2003) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Polyclonal Antibodies