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Nitric Oxide & NOS Detection / Related Products
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ALX-620-054 Revised 11-Oct-06
4AF DA
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SYNONYMS 4-Aminofluorescein diacetate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-054-M001   1 mg 136.00 USD Add To Cart
Product Specification
FORMULA: C24H17NO7
MW: 431.4
PURITY: ≥99% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light. Keep under inert gas.

Product Description
Negative control compound for the fluorescent probes DAF-2 (Prod. No. ALX-620-052) and DAF-2 DA (Prod. No. ALX-620-056).
Product Specific Literature References
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-850-323 Revised 31-Jul-08
ADMA Direct (human) ELISA Kit
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-850-323-KI01   1 Kit 1'100.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.05μmol/l (expected value: 0.45µmol/l ± 0.19µmol/l)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative detection of asymmetrical dimethylarginine (ADMA) in human EDTA-plasma and serum. Does not cross-react with SDMA, NMMA or L-arginine.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Store standard and controls at -20°C. Store rest of the kit at +4°C.
General Information
Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide (NO) synthesis. NO is one of the major endothelium-derived vasoactive mediators and is involved in the modulation of blood flow and blood pressure. Even small changes of ADMA concentration alter vascular NO production, vascular tone, and systemic vascular resistence. Elevated ADMA concentrations are found in patients with diabetes mellitus, hypercholeste-rolemia, hypertension, pulmonary hypertension, peripheral arterial disease, chronic renal failure, preeclampsia or erectile dysfunction. ADMA is therefore a novel cardiovascular risk factor.
MANUFACTURER Manufactured by Immundiagnostik AG.
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ELISA & EIA Kits • PRMTs, Mono- & Dimethylarginine / Related Products
 
 
ALX-850-327 Revised 31-Jul-08 New product
ADMA Direct (mouse/rat) ELISA Kit
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-850-327-KI01   1 Kit 1'400.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.05μmol/l
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative detection of asymmetrical dimethylarginine (ADMA) in mouse or rat EDTA-plasma and serum. Does not cross-react with SDMA, NMMA or L-arginine.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Store standard and controls at -20°C. Store rest of the kit at +4°C.
General Information
Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide (NO) synthesis. NO is one of the major endothelium-derived vasoactive mediators and is involved in the modulation of blood flow and blood pressure. Even small changes of ADMA concentration alter vascular NO production, vascular tone, and systemic vascular resistence. Elevated ADMA concentrations are found in patients with diabetes mellitus, hypercholeste-rolemia, hypertension, pulmonary hypertension, peripheral arterial disease, chronic renal failure, preeclampsia or erectile dysfunction. ADMA is therefore a novel cardiovascular risk factor.
MANUFACTURER Manufactured by Immundiagnostik AG.
Related Products
Further Categories Containing This Product:
ELISA & EIA Kits • PRMTs, Mono- & Dimethylarginine / Related Products
 
 
ALX-850-321 Revised 25-Feb-08
ADMA ELISA Kit - Replaced by Prod. No. ALX-850-323
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PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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not sold in USA
General Information
MANUFACTURER Manufactured by Immundiagnostik AG.
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ELISA & EIA Kits
 
 
ALX-620-052 Revised 25-Sep-08
DAF-2
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SYNONYMS 4,5-Diaminofluorescein
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-052-M001   1 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C20H16N2O5
MW: 364.3
CONCENTRATION: ~5mM
PURITY: ≥98%
FORMULATION: Pale yellow liquid. In 0.55ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Keep under inert gas. After opening, prepare aliquots and store at -20°C. Protect from light.
HAZARD: IRRITANT.

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Product Description
Highly sensitive fluorescent probe for the real-time detection of nitric oxide (NO) in vivo. In the cell, the product reacts rapidly with NO in the presence of O2 to form the highly fluorescent compound triazolofluorescein (DAF-2T (Prod. No. ALX-620-060), Ex 495nm; Em 515nm). Not for sale in Japan.
Product Specific Literature References
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998) Abstract
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) Abstract
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000) Abstract
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000) Abstract
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001) Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001) Abstract
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001) Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-056 Revised 25-Sep-08
DAF-2 DA (cell permeable)
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SYNONYMS 4,5-Diaminofluorescein . diacetate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-056-M001   1 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C24H18N2O7
MW: 446.4
CONCENTRATION: ~5mM
PURITY: ≥99%
FORMULATION: Liquid. In 0.45ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Keep under inert gas. Protect from light. After opening, prepare aliquots and store at -20°C.

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Product Description
Cell permeable analog of DAF-2 (Prod. No. ALX-620-052) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in nitric oxide (NO) levels in vivo (Ex(max): 495nm; Em(max): 515nm). Not for sale in Japan.
Product Specific Literature References
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) Abstract
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998) Abstract
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000) Abstract
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000) Abstract
Fluroescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001) Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001) Abstract
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001) Abstract
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J. Biol. Chem. 277, 25823 (2002) Abstract; Full Text
BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube: A. Traister, et al.; Cell Death Differ. (2004) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-060 Revised 25-Sep-08
DAF-2T
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SYNONYMS Diaminofluorescein-2T
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-060-C100   100 µg 160.00 USD Add To Cart
Product Specification
FORMULA: C20H11N3O5
MW: 373.3
CONCENTRATION: ~0.5mM
PURITY: ≥98% (HPLC)
FORMULATION: Pale yellow liquid. In 0.54ml DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
USE/STABILITY: Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: After opening, prepare aliquots and store at -20°C. Protect from light. Keep under inert gas.

Product Images
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Product Description
Fluorescent compound (Ex(max): 495nm; Em(max): 515nm) produced by the reaction between DAF-2 (Prod. No. ALX-620-052) and nitric oxide (NO). Control compound for NO detection using DAF-2 (Prod. No. ALX-620-052) or DAF-2 DA (cell permeable) (Prod. No. ALX-620-056). Not for sale in Japan.
Product Specific Literature References
Fluorescent indicators for imaging nitric oxide production: H. Kojima, et al.; Angew. Chem. Int. Ed. Engl. 38, 3209 (1999) Abstract
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-070 Revised 25-Sep-08
DAF-FM
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SYNONYMS Diaminofluorescein-FM
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-070-M001   1 mg 495.00 USD Add To Cart
Product Specification
FORMULA: C21H14F2N2O5
MW: 412.3
CONCENTRATION: ~7mM
PURITY: ≥98% (HPLC)
FORMULATION: Liquid. In 0.35ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Prepare 1’000-fold dilution (~7µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.

Product Description
Highly sensitive fluorescent probe (Ex: 500nm; Em: 515nm) for the detection of nitric oxide (NO). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Photostable. Not for sale in Japan.
Product Specific Literature References
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry: Y. Itoh, et al.; Anal. Biochem. 287, 203 (2000) Abstract
Fluorescent indicators for nitric oxide: H. Kojima & N. Nagano; Advanced Materials 12, 763 (2000)
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001) Abstract
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia: H. Kojima, et al.; J. Neurochem. 76, 1404 (2001) Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-071 Revised 25-Sep-08
DAF-FM DA (cell permeable)
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SYNONYMS Diaminofluorescein-FM . diacetate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-620-071-M001   1 mg 495.00 USD Add To Cart
Product Specification
FORMULA: C25H18F2N2O7
MW: 496.4
CONCENTRATION: ~5mM
PURITY: ≥98% (HPLC)
FORMULATION: Liquid. In 0.4ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Protect from light. Keep under inert gas. After opening, prepare aliquots and store at -20°C.

Product Description
Cell permeable analog of DAF-FM (Prod. No. ALX-620-070), that is hydrolyzed to DAF-FM by intracellular esterases (Ex: 500nm; Em: 515nm). Stable in a pH range of 5.5-12. High yield (quantum efficiency). Detection limit 3nM. Photostable. Not for sale in Japan.
Product Specific Literature References
Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry: Y. Itoh, et al.; Anal. Biochem. 287, 203 (2000) Abstract
Fluorescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia: H. Kojima, et al.; J. Neurochem. 76, 1404 (2001) Abstract
Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore: H. Kojima, et al.; Anal. Chem. 73, 1967 (2001) Abstract
Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe: S. Lepiller et al.; Free Radic. Biol. Med. 43, 619 (2007) Abstract
Further Categories Containing This Product:
Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-610-023 Revised 10-Jul-08
DAN-1 EE . hydrochloride
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SYNONYMS 4-((3-Amino-2-naphthalenyl)aminomethyl)benzoic acid ethyl ester . HCl
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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ALX-610-023-M005   5 mg 162.00 USD Add To Cart
Product Specification
FORMULA: C20H20N2O2 . HCl
MW: 320.4 . 36.5
PURITY: ≥95%
FORMULATION: Liquid. Solution in ethanol.
SOLUBILITY: 30mg/ml soluble in DMSO or dimethyl formamide; slightly soluble in PBS, pH 7.2 (0.5mg/ml).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.

Product Description
Fluorescent indicator for the bioimaging of nitric oxide (NO). Upon entry into the cell, DAN-1 EE is transformed by cytosolic esterases into the less cell permeable DAN-1. Intracellular formation of NO can be monitored (Ex(max): 360-380nm; Em(max): 420-450nm).
Product Specific Literature References
Development of a fluorescent indicator for the bioimaging of nitric oxide: H. Kojima, et al.; Biol. Pharm. Bull. 20, 1229 (1997) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION To change the solvent, evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice.
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Dyes / Stains / Fluorescent Probes / Fluorescent Labels
 
 
ALX-620-067 Revised 25-Sep-08
DAR-4M
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SYNONYMS Diaminorhodamine-4M
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection / Related Products
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