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Ras Signalling Pathway
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ALX-210-380 Revised 30-Oct-06
Polyclonal Antibody to β-Pix
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SYNONYMS anti-p85SPR PAb
anti-PAK-interacting Exchange Factor β PAb
anti-Rho Guanine Nucleotide Exchange Factor 7 PAb
anti-COOL-1 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Guanine-nucleotide Exchange Factors [GEFs]
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-380-C100   100 µg 290.00 USD Add To Cart
ALX-210-380B-C050 Biotin 50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant β-Pix (PAK-interacting exchange factor β) SH3 domain.
SPECIFICITY: Recognizes the multiple splice variants of human, mouse and rat β-Pix-a, -b, bL, -c and -d isoforms.
APPLICATION: ELISA (direct and indirect: 1:5'000-1:10'000)
Immunocytochemistry (1:1'000)
Western Blot (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion: W.K. Oh, et al.; BBRC 235, 794 (1997) Abstract
betaPix-b(L), a novel isoform of betaPix, is generated by alternative translation: S. Rhee, et al.; BBRC 318, 415 (2004) Abstract
General Information
β-Pix is a p21-activated protein kinase (Pak)-interacting exchange factor that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. β-Pix is expressed ubiquitously in all the tissue examined and other isoforms are expressed mainly in the central nervous system.
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
A novel regulator of p21-activated kinases: S. Bagrodia, et al.; J. Biol. Chem. 273, 23633 (1998) Abstract
Leucine zipper-mediated homodimerization of the p21-activated kinase-interacting factor, beta Pix. Implication for a role in cytoskeletal reorganization: S. Kim, et al.; J. Biol. Chem. 276, 10581 (2001) Abstract
Human immunodeficiency virus type 1 Nef selectively associates with a catalytically active subpopulation of p21-activated kinase 2 (PAK2) independently of PAK2 binding to Nck or beta-PIX: G.H. Renkema, et al.; J. Virol. 75, 2154 (2001) Abstract
Genomic structure of the human beta-PIX gene and its alteration in gastric cancer: Z. Li, et al.; Cancer Lett. 177, 203 (2002) Abstract
Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific guanine nucleotide exchange factor, via the Ras/ERK/PAK2 pathway is required for basic fibroblast growth factor-induced neurite outgrowth: E.Y. Shin, et al.; J. Biol. Chem. 277, 44417 (2002) Abstract
Mammalian Scribble forms a tight complex with the betaPIX exchange factor: S. Audebert, et al.; Curr. Biol. 14, 987 (2004) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-793 Revised 12-Jan-05
Polyclonal Antibody to Rab 3A
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Rab / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-793-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-18 (M1ASATDSRYGQKESSDQN18C) of human Rab 3A.
SPECIFICITY: Recognizes human, mouse, rat, hamster and dog Rab 3A. Detects a band of ~21kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Exocytosis • Polyclonal Antibodies
 
 
ALX-210-794 Revised 12-Jan-05
Polyclonal Antibody to Rab 3C (mouse)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Rab / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-794-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Purified antibody in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 9-26 (M9ASAQDARFGQ
KDSSDQN26C) of rat Rab 3C.
SPECIFICITY: Recognizes mouse Rab 3C. Detects a band of ~22kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Further Categories Containing This Product:
Vesicles & Membrane Trafficking Other Products • Polyclonal Antibodies
 
 
ALX-210-855 Revised 09-Aug-07
Polyclonal Antibody to Rab 3D (mouse)
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Rab / Related Products
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ALX-210-855-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST: From rabbit.
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-18 (M1ASASEPPASPRDAADQN18) of rat Rab 3D. This sequence is completely conserved in mouse Rab 3D.
SPECIFICITY: Recognizes mouse Rab 3D. Detects a band of ~29kDa by Western blot. Does not cross-react with Rab 3A, 3B or 3C.
APPLICATION: Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-166-008.
Further Categories Containing This Product:
Vesicles & Membrane Trafficking Other Products • Polyclonal Antibodies
 
 
ALX-210-781 Revised 16-Feb-05
Polyclonal Antibody to RasGAP (CT)
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SYNONYMS anti-Ras-specific GTPase-activating Protein (CT) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
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ALX-210-781-R100   100 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. 100µl serum containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 1034-1047 of human RasGAP (Ras-specific GTPase-activating protein).
SPECIFICITY: Recognizes human, mouse, rat and monkey full-length RasGAP as well as the C-terminal (CT) fragment generated by caspase cleavage (RasGAP aa 456-1047). Detects a band of ~150 kDa by Western blot. Does not cross-react with the N-terminal portion of RasGAP (including the SH2 and SH3 domains).
APPLICATION: Immunoprecipitation (1:1'000; only CT RasGAP; see technical note)
Western Blot (1:1'000-1:3'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
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Product Description
RasGAP
RasGAP (Ras-specific GTPase-activating protein) is one of the few signalling proteins cleaved by caspases during apoptosis [1-3]. The kinetics of RasGAP cleavage is identical to well-known caspase substrates such as PARP or the caspase themselves. RasGAP cleavage by caspases generates a carboxy-terminal fragment of about 64 kDa (fragment C) containing the GAP activity and a N-terminal fragment (fragment N) of about 56 kDa. The latter fragment can be further cleaved into two other smaller fragments, one of which contains the SH2 and SH3 domains. The fact that RasGAP is an abundant ubiquitous protein readily cleaved during apoptosis makes it a very good apoptotic marker.
RasGAP is a unique caspase substrate because it can either inhibit or promote apoptosis depending on the extent of its cleavage by caspases. At low levels of caspase activity, generation of fragment N induces a potent Ras-PI3K-Akt-dependent survival pathway. At higher levels of caspase activity, fragment N is further cleaved into two fragments (N1 and N2) that potentiate apoptosis. RasGAP has recently been qualified as an “apoptostat” because it functions as a sensor of caspase activity to determine whether or not a cell should survive [2].
Product Specific Literature References
[1] Caspase-dependent cleavage of signaling proteins during apoptosis. A turn-off mechanism for anti-apoptotic signals: C. Widmann, et al.; J. Biol. Chem. 273, 7141 (1998) Abstract; Full Text
[2] Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP: J.-Y. Yang, et al.; Mol. Cell. Biol. 21, 5346 (2002) Abstract; Full Text
[3] The RasGAP N-terminal fragment generated by caspase cleavage protects cells in a Ras/PI3K/Akt-dependent manner that does not rely on NF-κB activation: J.-Y. Yang, et al.; J. Biol. Chem. 277, 14641 (2002) Abstract; Full Text
Ras GTPase-activating protein binds to Akt and is required for its activation: Y. Yue, et al.; J. Biol. Chem. 279, 12883 (2004) Abstract; Full Text
RasGTPase-activating protein is a target of caspases in spontaneous apoptosis of lung carcinoma cells and in response to etoposide: B. Bartling, et al.; Carcinogenesis 25, 909 (2004) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Technical note: Due to the conformation of native full-length RasGAP, steric hinderance prevents binding of this antibody to the C-terminus. Thus immunoprecipitation is possible only with the C-terminal RasGAP fragment and not with the full-length protein.

The C-terminal (aa 456-1047) fragment contains the GTPase-activating protein activity of RasGAP as well as a C2 domain and a PH domain.
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other Products • Polyclonal Antibodies
 
 
ALX-210-860 Revised 31-Oct-06
Polyclonal Antibody to RasGAP (NT)
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SYNONYMS anti-Ras-specific GTPase-activating Protein (NT) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-860-R100   100 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: Affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RasGAP (Ras-specific GTPase-activating protein) fragment N2 (aa 158-455).
SPECIFICITY: Recognizes human, mouse and rat full-length RasGAP as well as the N-terminal fragment (that comprises SH2 and SH3 domains) generated by caspase cleavage (fragments N and N2). Does not cross-react with other GAP proteins.
APPLICATION: Immunoprecipitation
Western Blot (1:1'000-1:3'000 for detection of the full-length protein; 1:100-1:300 for detection of the caspase-generated fragments).
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
[1] Antiapoptotic signaling generated by caspase-induced cleavage of RasGAP: J.-Y. Yang, et al.; Mol. Cell. Biol. 21, 5346 (2001) Abstract; Full Text
[2] RasGTPase-activating protein is a target of caspases in spontaneous apoptosis of lung carcinoma cells and in response to etoposide: B. Bartling, et al.; Carcinogenesis 25, 909 (2004) Abstract
General Information
RasGAP (Ras-specific GTPase-activating protein) is one of the few signalling proteins cleaved by caspases during apoptosis [1,3,4]. The kinetics of RasGAP cleavage is identical to well-known caspase substrates such as PARP or the caspase themselves. RasGAP cleavage by caspases generates a C-terminal fragment of about 64kDa (fragment C) containing the GAP activity and a N-terminal fragment (fragment N) of about 56kDa. The latter fragment can be further cleaved into two other smaller fragments, one of which contains the SH2 and SH3 domains. The fact that RasGAP is an abundant ubiquitous protein readily cleaved during apoptosis makes it a very good apoptotic marker.
RasGAP is a unique caspase substrate because it can either inhibit or promote apoptosis depending on the extent of its cleavage by caspases. At low levels of caspase activity that do not induce apoptosis, generation of fragment N induces a potent Ras-PI3K-Akt-dependent survival pathway [5]. The formation of fragment N is mandatory for survival of stressed cells. The presence of fragment N, but not of fragment N2, in cells is indicative of mild caspase-3 activity. At higher levels of caspase activity, fragment N is further cleaved into two fragment (N1 and N2) that potentiate apoptosis. The presence of fragment N2 in cells is indicative of high caspase-3 activity. RasGAP has recently been qualified as an “apoptostat” because it functions as a sensor of caspase activity to determine whether or not a cell should survive [1].
General Literature References
[3] Caspase-dependent cleavage of signaling proteins during apoptosis. A turn-off mechanism for anti-apoptotic signals: C. Widmann, et al.; J. Biol. Chem. 273, 7141 (1998) Abstract; Full Text
[4] Proteolytic cleavage of ras GTPase-activating protein during apoptosis: L.P. Wen, et al.; Cell Death Differ. 5, 729 (1998) Abstract
[5] The RasGAP N-terminal fragment generated by caspase cleavage protects cells in a Ras/PI3K/Akt-dependent manner that does not rely on NFkappa B activation: J.Y. Yang and C. Widmann; J. Biol. Chem. 277, 14641 (2002) Abstract; Full Text
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other Products • Polyclonal Antibodies
 
 
ALX-215-062 Revised 16-Jul-08 New product
Polyclonal Antibody to Rho-related BTB Domain-containing Protein 2
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SYNONYMS anti-RhoBTB2/DBC2 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Rho / Related Products
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ALX-215-062-C050   50 µg 140.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Affinity purified using the immunogenic peptide.
FORMULATION: Liquid. In PBS containing 0.1% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to the conserved human Rho-related BTB domain-containing protein 2 amino acids 673-687 (KEEDHYQRARKEREK) with an N-terminal C-residue.
SPECIFICITY: Recognizes human, mouse, rat, chicken and zebrafish Rho-related BTB domain-containing protein 2.
APPLICATION: Immunocytochemistry (1:40)
Western Blot (1:200)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
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Product Specific Literature References
RhoBTB2 (DBC2) is a mitotic E2F1 target gene with a novel role in apoptosis: S.N. Freeman, et al.; J. Biol. Chem. 283, 2353 (2008) Abstract; Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products • Polyclonal Antibodies
 
 
ALX-210-022 Revised 29-Jun-05
Polyclonal Antibody to R-Ras
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SYNONYMS anti-Ras-related Protein R-Ras PAb
anti-p23 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Ras / Related Products
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ALX-210-022-R050   50 µl 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 11-31 (R11GRPRGGGPGPRD
PPPGETHK31) of mouse  Ras-related protein R-Ras.
SPECIFICITY: Recognizes human and mouse R-Ras.
APPLICATION: Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -80°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
R-Ras promotes apoptosis caused by growth factor deprivation via a Bcl-2 suppressible mechanism: H.G. Wang, et al.; J. Cell. Biol. 129, 1103 (1995) Abstract; Full Text
Integrin activation by R-ras: Z. Zhang, et al.; Cell 85, 61 (1996) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-535 Revised 31-Oct-06
Polyclonal Antibody to Shc
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SYNONYMS anti-Shc Transforming Protein 1 PAb
anti-SH2 Domain Protein C1 PAb
anti-Shc1 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY SH2 & SH3 Domains
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ALX-210-535-R100   100 µl 220.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
PURITY: Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human Shc portion (aa 1180-1620).
SPECIFICITY: Recognizes human, mouse and hamster p46shc, p52shc and p66shc isoforms of phosphorylated and unphosphorylated Shc.
APPLICATION: ELISA
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
There are 3 alternatively spliced isoforms of the Shc adapter pro-tein: p46SHC, p52SHC and p66SHC, that link activated growth factor receptors to signalling pathway. Isoform p46SHC and p52SHC, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS com-plex and are implicated in mitogenic signalling. They function as initiators of the Ras signalling cascade in various non-neuronal systems. The isoform p66SHC does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. It acts as a downstream target of the tumor suppressor p53 and is indispen-sable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis.
Further Categories Containing This Product:
Phospho-specific Antibodies • Shc & Other Ras Adaptors / Related Products • Polyclonal Antibodies
 
 
ALX-210-269 Revised 13-Jan-05
Polyclonal Antibody to SynGAP
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SYNONYMS anti-Synaptic Ras-GTPase Activating Protein PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY GTPase-activating Proteins [GAPs]
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-269-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 947-1167 of rat SynGAP (synaptic Ras-GTPase activating protein).
SPECIFICITY: Recognizes mouse and rat SynGAP. Detects a band of ~135kDa by Western blot.
APPLICATION: Immunocytochemistry (1µg/ml)
Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
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