ALEXIS Biochemicals: Phorbol 12,13-didecanoate

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Ecdysteroids

Microcystins

Natural Products - ATPase Inhibitors

Natural Products - NF-kB Pathway Inhibitors

Natural Products - Protein Kinase Inhibitors

Okadaic Acids

Phorbols / Phorbol Esters

Natural Products - Other Signal Transduction Pathway Modulators

ALX-370-010

Revised 21-May-08

Muristerone A
SYNONYMS	2β,3β,5β,11α,14α,20R,22R-Heptahydroxycholest-7-en-6-one
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Ecdysteroids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-010-C100		100 µg	28.00 USD
ALX-370-010-C250		250 µg	50.00 USD
ALX-370-010-C500		500 µg	80.00 USD
ALX-370-010-M001		1 mg	140.00 USD

Product Specification

FORMULA:	C₂₇H₄₄O₈
MW:	496.6
CAS NUMBER:	38778-30-2
PURITY:	≥94%
APPEARANCE:	White to off-white crystals.
SOLUBILITY:	Soluble in methanol, 100% ethanol, acetic acid or DMSO. Insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Ecdysteroid. Due to reduced availability we recommend the alternative use of ponasterone A (Prod. No. ALX-370-014).

Product Specific Literature References

Structure of muri-sterone A, a new phytoecdysone: L. Canonica, et al.; J. C. S. Chem. Commun. 1060 (1972)
A novel method of isolation of phytoecdysones from kaladana seeds: L. Canonica, et al.; Phytochemistry 14, 525 (1975)
New phytoecdysones from kaladana. I. Structure of muristerone A and kaladasterone: L. Canonica, et al.; Gazz. Chim. Ital. 107, 123 (1977)
Ecdysone-inducible gene expression in mammalian cells and transgenic mice: D. No, et al.; PNAS 93, 3346 (1996) Abstract
The ecdysone inducible gene expression system: unexpected effects of muristerone A and ponasterone A on cytokine signaling in mammalian cells: S. Constantino, et al.; Eur. Cytokine Netw. 12, 365 (2001) Abstract
Selected technologies to control genes and their products for experimental and clinical purposes: H.K. Alexander, et al.; Arch. Immunol. Ther. Exp. (Warsz.) 55, 139 (2007), Review Abstract

Further Categories Containing This Product:

Expression Systems / Related Products

ALX-370-011

Revised 07-Jan-08

Ecdysone
SYNONYMS	α-Ecdysone 2β,3β,14α,22R,25-Pentahydroxy-5β-cholest-7-en-6-one
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Ecdysteroids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-011-M001		1 mg	48.00 USD
ALX-370-011-M005		5 mg	150.00 USD
ALX-370-011-M010		10 mg	240.00 USD

Product Specification

FORMULA:	C₂₇H₄₄O₆
MW:	464.6
CAS NUMBER:	3604-87-3
MERCK INDEX:	14: 3491
PURITY:	≥95%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in methanol, 100% ethanol, acetic acid or DMSO. Sparingly soluble in chloroform. Insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Ecdysteroid first described as moulting hormone in insects.

Product Specific Literature References

P. Karlon, et al.; J. Liebig's Ann. Chem. 662, 1 (1963)
[Ecdysone as companion substance of ecdysterone in Polypodium vulgare L]: G. Heinrich & H. Hoffmeister; Experientia 23, 995 (1967), (Article in German) Abstract
L. Canonica, et al.; Phytochemistry 14, 525 (1975)
Hormones, puffs and flies: the molecular control of metamorphosis by ecdysone: A.J. Andres & C.S. Thummel; Trends Genet. 8, 132 (1992), Review Abstract
Ecdysone signaling cascade and regulation of Drosophila metamorphosis: E.H. Baehrecke; Arch. Insect Biochem. Physiol. 33, 231 (1996), Review Abstract

Further Categories Containing This Product:

Expression Systems / Related Products

ALX-370-012

Revised 09-Oct-07

20-Hydroxyecdysone
SYNONYMS	β-Ecdysone 2β,3β,14α,20R,22R,25-Hexahydroxy-5β-cholest-7-en-6-one Ecdysterone
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Ecdysteroids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-012-M005		5 mg	55.00 USD
ALX-370-012-M010		10 mg	90.00 USD
ALX-370-012-M050		50 mg	350.00 USD

Product Specification

FORMULA:	C₂₇H₄₄O₇
MW:	480.6
CAS NUMBER:	5289-74-7
MERCK INDEX:	14: 3491
PURITY:	≥95%
APPEARANCE:	White powder.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Most widely occurring ecdysteroid in both plant and animal species. Controls cell death during metamorphosis of Drosophila melanogaster.

Product Specific Literature References

H. Hampshire and D.H.S. Horn; Chem. Commun. 37 (1966)
M.N. Galbraith and D.H.S. Horn; Chem. Commun. 905 (1966)
H. Rimpler and G. Schulz; THL 22, 2033 (1967)
L. Canonica, et al.; Phytochemistry 14, 525 (1975)
Fork head controls the timing and tissue selectivity of steroid-induced developmental cell death: C. Cao, et al.; J. Cell Biol. 176, 843 (2007) Abstract

Further Categories Containing This Product:

Forkhead Box [FOX] Proteins / Related Products • Non-apoptotic Cell Death / Necrosis

ALX-370-013

Revised 07-Jan-08

Makisterone A
SYNONYMS	2β,3β,14α,20R,22R,25-Hexahydroxy-5β-24R-ergost-7-en-6-one
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Ecdysteroids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-013-C250		250 µg	38.00 USD
ALX-370-013-M001		1 mg	105.00 USD

Product Specification

FORMULA:	C₂₈H₄₆O₇
MW:	494.7
CAS NUMBER:	20137-14-8
PURITY:	≥95%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in methanol, 100% ethanol, acetic acid or DMSO. Sparingly soluble in chloroform. Insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Ecdysteroid.

Product Specific Literature References

The determination of absolute configuration at C-24 of the phytoecdysone makisterone A: B. Danieli, et al.; J. C. S. Chem. Commun. 745 (1974)
A novel method of isolation of phytoecdysones from kaladana seeds: L. Canonica, et al.; Phytochemistry 14, 525 (1975)
Evidence for the presence of makisterone A in Drosophila larvae and the secretion of 20-deoxymakisterone A by the ring gland: C.P. Redfern; PNAS 81, 5643 (1984) Abstract
Ecdysteroids increase the yield of recombinant protein produced in baculovirus insect cell expression system: M. Sarvari, et al.; BBRC 167, 1154 (1990) Abstract
Ecdysteroids of quinoa seeds (Chenopodium quinoa Willd.): N. Zhu, et al.; J. Agric. Food Chem. 49, 2576 (2001) Abstract

Further Categories Containing This Product:

Expression Systems / Related Products

ALX-370-014

Revised 16-May-08

Ponasterone A
SYNONYMS	25-Deoxy-20-hydroxyecdysone 25-Deoxyecdysterone 2β,3β,14α,20R,22R-Pentahydroxy-5β-cholest-7-en-6-one
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Ecdysteroids

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-370-014-M001		1 mg	45.00 USD
ALX-370-014-M005		5 mg	180.00 USD
ALX-370-014-M100		100 mg	2'800.00 USD

Product Specification

FORMULA:	C₂₇H₄₄O₆
MW:	464.6
CAS NUMBER:	13408-56-5
MERCK INDEX:	14: 7588
SOURCE/HOST:	Synthetic.
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in acetic acid, 100% ethanol, methanol or DMSO; insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	To prepare a 1mM stock solution (1.1mM) in 100% ethanol: Add 2ml of 100% ethanol to 1mg (2µmol). Gently mix to dissolve the product (5 to 10 min.). Do not heat solution to dissolve the product. Store the 1mM stock solution at +4°C or at -20°C.

Product Description

A member of the ecdysteroid family. Analog of ecdysone (Prod. No. ALX-370-011) with similar properties to muristerone A (Prod. No. ALX-370-010). It is a functional, reliable and economical substitute for muristerone A as an inducer for the ecdysone-inducible mammalian expression system. Results show that ponasterone A was able to induce expression of β-galactosidase to levels similar to those obtained with muristerone A induction.

Product Specific Literature References

K. Nakanishi, et al.; Chem. Commun. 915 (1966)
H. Moriyama, et al.; THL 9, 1111 (1968)
K. Nakanishi, et al.; Bull. Soc. Chim. France 3475 (1969), (Review)
The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster: C. Beckers, et al.; Mol. Cell. Endocrinol. 17, 51 (1980) Abstract
Past and present studies with ponasterones, the first insect molting hormones from plants: K. Nakanishi; Steroids 57, 649 (1992) Abstract
Identification of ligands and coligands for the ecdysone-regulated gene switch: E. Saez, et al.; PNAS 97, 14512 (2000) Abstract
Recent advances in inducible expression in transgenic mice: C. Albanese, et al.; Semin. Cell. Dev. Biol. 13, 129 (2002) Abstract
Development of a ponasterone A-inducible gene expression system for application in cultured skeletal muscle cells: Y.Y. Xiao, et al.; Int. J. Biochem. Cell. Biol. 35, 79 (2003) Abstract

Further Categories Containing This Product:

Expression Systems / Related Products

ALX-350-012

Revised 23-Oct-08

Microcystin-LR
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Microcystins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-012-C050		50 µg	24.00 USD
ALX-350-012-C100		100 µg	46.00 USD
ALX-350-012-C500		500 µg	175.00 USD
ALX-350-012-M001		1 mg	330.00 USD

Product Specification

FORMULA:	C₄₉H₇₄N₁₀O₁₂
MW:	995.2
CAS NUMBER:	101043-37-2
RTECS:	GT2810000
SOURCE/HOST:	Isolated from Microcystis aeruginosa.
PURITY:	≥95% (HPLC)
APPEARANCE:	Whitish film adhered to inside of the vial.
SOLUBILITY:	Soluble in 100% ethanol, methanol or DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stock solutions are stable for up to 6 months when stored at -20°C. Unstable at pH>7.7.
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap.
HAZARD:	MAY BE CARCINOGENIC. VERY TOXIC. HIGHLY IRRITANT.

Product Description

Heptapeptide ester hepatotoxin. Tumor promoter. Equally potent and selective inhibitor of protein phosphatase 1 (PP1) and 2A (PP2A). PP2B is less sensitive and PP2C is not inhibited up to 4µM. Useful for affinity-purification of PP2A. The product is not cell permeable except in liver cells, which appear to have a functional uptake system. Has no effect on protein kinases. Less toxic than the more hydrophobic analogs microcystin-LY, -LW and -LF. Frequently contaminates fresh-water lakes and ponds. Causes livestock poisonings. Ozonation did lead to complete loss of toxicity and toxins from contaminated samples.

Product Specific Literature References

Structural studies on cyanoginosins-LR, -YR, -YA, and -YM, peptide toxins from Microcystis aeruginosa: D.P. Botes, et al.; JCS Perkin Trans. I, 2747 (1985)
Nodularin, microcystin, and the configuration of Adda: K.L. Rinehart, et al.; JACS 110, 8557 (1988)
Characterization of microcystin-LR, a potent inhibitor of type 1 and type 2A protein phosphatases: R.E. Honkanen, et al.; J. Biol. Chem. 265, 19401 (1990) Abstract; Full Text
Cyanobacterial microcystin-LR is a potent and specific inhibitor of protein phosphatases 1 and 2A from both mammals and higher plants: C. MacKintosh, et al.; FEBS Lett. 264, 187 (1990) Abstract
Protein phosphatase 2A is a specific protamine-kinase-inactivating phosphatase: G.D. Amick, et al.; Biochem. J. 287, 1019 (1992) Abstract
Evidence for the regulation of exocytic transport by protein phosphorylation: H.W. Davidson, et al.; J. Cell. Biol. 116, 1343 (1992) Abstract
Liver tumor promotion by the cyanobacterial cyclic peptide toxin microcystin-LR: R. Nishiwaki-Matsushima, et al.; J. Cancer Res. Clin. Oncol. 118, 420 (1992) Abstract
Two significant aspects of microcystin-LR: specific binding and liver specificity: R. Nishiwaki, et al.; Cancer Lett. 83, 283 (1994) Abstract
Comparative toxicity of four microcystins of different hydrophobicities to the protozoan, Tetrahymena pyriformis: C.J. Ward & G.A. Codd; J. Appl. Microbiol. 86, 874 (1999) Abstract
Unique features of the okadaic acid activity class of tumor promoters: H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol. 125, 150 (1999), Review Abstract
The toxicology of microcystin-LR: occurrence, toxicokinetics, toxicodynamics, diagnosis and treatment: K. Bischoff; Vet. Hum. Toxicol. 43, 294 (2001), Review Abstract
Negative regulation of ERK and Elk by protein kinase B modulates c-Fos transcription: I. Galetic, et al.; J. Biol. Chem. 278, 4416 (2003) Abstract; Full Text
Decrease in toxicity of microcystins LA and LR in drinking water by ozonation: S. Brooke, et al.; Toxicon. 48, 1054 (2006) Abstract
Mitochondria a key role in microcystin-LR kidney intoxication: R. La-Salete, et al.; J. Appl. Toxicol. 28, 55 (2008) Abstract

ALX-850-319	Microcystins (Adda specific) ELISA Kit
ALX-350-043	Microcystin-RR
ALX-350-148	Microcystin-LY
ALX-350-080	Microcystin-LW
ALX-350-081	Microcystin-LF
ALX-350-096	Microcystin-LA
ALX-350-044	Microcystin-YR
ALX-804-320	Monoclonal Antibody to Microcystin-LR (MC10E7)
ALX-804-585	Monoclonal Antibody to Microcystins (Adda specific) (AD4G2)

ALX-350-044	Microcystin-YR
ALX-804-585	Monoclonal Antibody to Microcystins (Adda specific) (AD4G2)
ALX-350-148	Microcystin-LY
ALX-850-319	Microcystins (Adda specific) ELISA Kit
ALX-350-080	Microcystin-LW
ALX-350-012	Microcystin-LR
ALX-350-081	Microcystin-LF
ALX-350-096	Microcystin-LA

FORMULA:	C₄₉H₇₅N₁₃O₁₂
MW:	1038.2
CAS NUMBER:	111755-37-4
SOURCE/HOST:	Isolated from Microcystis aeruginosa.
PURITY:	≥95% (HPLC)
APPEARANCE:	Clear to whitish film adhered to inside of vial.
SOLUBILITY:	Soluble in 80% aqueous methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap.
HAZARD:	MAY BE CARCINOGENIC. VERY TOXIC. HIGHLY IRRITANT.
IDENTITY:	Identity determined by MS.