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DNA Regulation / Transcription
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ALX-480-047 Revised 23-Jul-08
5,6-Dichloro-1-β-D-ribofuranosyl benzimidazole 3',5'-cyclic Monophosphothioate, Sp-Isomer . sodium salt
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SYNONYMS Sp-5,6-DCl-cBIMPS . Na
5,6-DCI-cBIMPS . Na, Sp-Isomer
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Other Cyclic Nucleotides
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-480-047-C100   100 µg 30.00 USD Add To Cart
ALX-480-047-M001   1 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C12H10Cl2N2O5PS . Na
MW: 396.2 . 23.0
CAS NUMBER: 120912-54-1
PURITY: ≥99%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO (167 mM), dimethyl formamide (167 mM), 96 % ethanol (167 mM), methanol (25 mM), water (25 mM) and aqueous buffers such as PBS (pH 7.4; 1.4 mM), 25 mM TRIS (pH 7.4-; 25 mM), 25 mM HEPES (pH 7.2; 35.7 mM) and Na2HPO4 (pH 7.0; 2.8 mM).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Potent, specific and site selective activator of cAMP-dependent protein kinases especially suitable for intact cells showing preference for site B of type II isozyme. Completely stable against mammalian phosphodiesterases type I and III and only extremely slowly hydrolyzed by type II. Surpasses the widely used but problematic dibutyryl-cAMP (Prod. No. ALX-480-045) or 8-CPT-cAMP (Prod. No. ALX-480-030) in terms of metabolic stability, membrane permeability and potency.
Product Specific Literature References
Characterization of Sp-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole- 3',5'-monophosphorothioate (Sp-5,6-DCl-cBiMPS) as a potent and specific activator of cyclic-AMP-dependent protein kinase in cell extracts and intact cells: M. Sandberg, et al.; Biochem J. 279, 521 (1991) Abstract
Further Categories Containing This Product:
PKA Activators
 
 
ALX-480-048 Revised 06-Mar-07
2',3'-Dideoxyadenosine
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Adenosine Derivatives Other Products
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ALX-480-048-M001   1 mg 15.00 USD Add To Cart
ALX-480-048-M005   5 mg 30.00 USD Add To Cart
Product Specification
FORMULA: C10H13N5O2
MW: 235.2
CAS NUMBER: 4097-22-7
MERCK INDEX: 14: 3101
PURITY: ≥97%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C

Product Description
Inhibitor of adenylate cyclase. Useful tool in antiviral and anticancer studies. Blocks viral reverse transcription from RNA to DNA. Inhibitor of adenosine deaminase.
Product Specific Literature References
Interpretation of the roles of adenylosuccinate lyase and of AMP deaminase in the anti-HIV activity of 2',3'-dideoxyadenosine and 2',3'- dideoxyinosine: V. Nair & T.B. Sells; Biochim. Biophys. Acta 1119, 201 (1992) Abstract
Long-term inhibn. of human T-lymphotropic virus type III/lymphadenopathy-associated virus DNA synthesis and RNA expression in T cells protected by 2',3'-dideoxynucleosides in vitro: H. Mitsuya, et al.; PNAS 84, 2033 (1987) Abstract
Further Categories Containing This Product:
Adenylyl Cyclase Inhibitors • Antiviral Agents Other Products
 
 
ALX-480-049 Revised 03-Jun-08
2',5'-Dideoxyadenosine
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PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Adenosine Derivatives Other Products
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ALX-480-049-M001   1 mg 30.00 USD Add To Cart
ALX-480-049-M005   5 mg 90.00 USD Add To Cart
Product Specification
FORMULA: C10H13N5O2
MW: 235.2
CAS NUMBER: 6698-26-6
PURITY: ≥98% (TLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water (20mg/ml at 50°C) or DMSO (30mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Solutions are stable for up to 3 months when stored at -20°C.

Product Description
Cell permeable inhibitor of adenylate cyclase (ID50=250µM [1]; inhibition also reported at 1mM [2]). Inhibits growth and causes cell differentiation (1.5µM) in K562 cells [6].
Product Specific Literature References
[1] Role of adenylate cyclase in immunologic release of mediators from rat mast cells: agonist and antagonist effects of purine- and ribose- modified adenosine analogs: S.T. Holgate, et al.; PNAS 77, 6800 (1980) Abstract
[2] Inhibition of adenylate cyclase attenuates adenosine receptor-mediated relaxation in coronary artery: M.H. Sabouni, et al.; J. Pharmacol. Exp. Ther. 259, 508 (1991) Abstract
[3] Direct inhibitory effect of neurotensin on isolated gastric smooth muscle cells of guinea pig via the cyclic GMP system: Y. Chijiiwa, et al.; Digestion 54, 135 (1993) Abstract
[4] Isoproterenol antagonistic effect of 2',5'-dideoxyadenosine in the isolated perfused guinea-pig heart: M. Hartmann & J. Schrader; J. Mol. Cell. Cardiol. 25, 331 (1993) Abstract
[5] Erythroid differentiation and growth inhibition of K562 cells by 2',5'-dideoxyadenosine: synergism with interferon-alpha: K. Ogawa, et al.; Leuk. Res. 19, 749 (1995) Abstract
Further Categories Containing This Product:
Adenylyl Cyclase Inhibitors • Antiviral Agents Other Products
 
 
ALX-400-003 Revised 25-May-07
Diethyldithiocarbamic acid . sodium salt . trihydrate (high purity)
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SYNONYMS DETC . 3H2O (high purity)
Diethyldithiocarbamate . 3H2O (high purity)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
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ALX-400-003-G005   5 g 17.00 USD Add To Cart
ALX-400-003-G025   25 g 27.00 USD Add To Cart
Product Specification
FORMULA: C5H10NS2 . Na . 3H2O
MW: 148.3 . 23.0 . 54.0
CAS NUMBER: 20624-25-3
MERCK INDEX: 14: 3378
RTECS: EZ6550000
PURITY: ≥99%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: HARMFUL.

Product Description
Nitric oxide (NO) spin-trapping reagent. Thiol and iron chelator. Inhibits induction of macrophage nitric oxide synthase (NOS). Has been shown to be an inhibitor of the nuclear transcription factor κB (NF-κB).
Product Specific Literature References
On-line detection of nitric oxide formation in liquid aqueous phase by electron paramagnetic resonance spectroscopy: P. Mordvintcev, et al.; Anal. Biochem. 199, 142 (1991) Abstract
NO accounts completely for the oxygenated nitrogen species generated by enzymic L-arginine oxygenation: A. Mülsch, et al.; Biochem. J. 288, 597 (1992) Abstract
Iron diethyldithiocarbamate as spin trap for nitric oxide detection: A.F. Vanin; Methods Enzymol. 301, 269 (1999), Review Abstract
Diethyldithiocarbamate inhibits induction of macrophage NO synthase: A. Mülsch, et al.; FEBS Lett. 321, 215 (1993) Abstract
The relationship between L-arginine-dependent nitric oxide synthesis, nitrite release and dinitrosyl-iron complex formation by activated macrophages: A. Vanin, et al.; Biochim. Biophys. Acta 1177, 37 (1993) Abstract
Dithiocarbamates as potent inhibitors of nuclear factor kappa B activation in intact cells: R. Schreck, et al.; J. Exp. Med. 175, 1181 (1992) Abstract
In vivo spin trapping of nitric oxide generated in the small intestine, liver, and kidney during the development of endotoxemia: a time-course study: G. Wallis, et al.; Shock 6, 274 (1996) Abstract
Epr evidence of nitric oxide production by the regenerating rat liver: M. Obolenskaya, et al.; BBRC 202, 571 (1994) Abstract
EPR and laser flash photolysis studies of the reaction of nitric oxide with water soluble NO trap Fe(II)-proline-dithiocarbamate complex: S.V. Paschenko, et al.; BBRC 225, 577 (1996) Abstract
Complexes of Fe2+ with diethyldithiocarbamate or N-methyl-D-glucamine dithiocarbamate as traps of nitric oxide in animal tissues: comparative investigations: V.D. Mikoyan, et al.; Biochim. Biophys. Acta 1336, 225 (1997) Abstract
NO-dependent mechanisms of adaptation to hypoxia: I.Y. Malyshev, et al.; Nitric Oxide 3, 105 (1999) Abstract
Ex vivo EPR detection of nitric oxide in brain tissue: H. Fujii & L.J. Berliner; Magn. Reson. Med. 42, 599 (1999) Abstract
EPR spectroscopy of common nitric oxide - spin trap complexes: S. Nedeianu & T. Pali; Cell Mol. Biol. Lett. 7, 142 (2002) Abstract
EPR quantification of vascular nitric oxide production in genetically modified mouse models: J.P. Khoo, et al.; Nitric Oxide 10, 156 (2004) Abstract
Evaluation of lipid-based carrier systems and inclusion complexes of diethyldithiocarbamate-iron to trap nitric oxide in biological systems: N. Charlier, et al.; Magn. Reson. Med. 55, 215 (2006) Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors • NOS Inhibitors (NOS Induction & Enzyme Activity)
 
 
ALX-280-017 Revised 31-Oct-06
Diethylmaleate
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SYNONYMS 2-Butenedioic acid
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NF-kB Pathway Inhibitors
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ALX-280-017-G005   5 g 15.00 USD Add To Cart
Product Specification
FORMULA: C8H12O4
MW: 172.2
CAS NUMBER: 141-05-9
MERCK INDEX: 14: 3123
RTECS: ON1225000
PURITY: ≥97%
APPEARANCE: Clear liquid.
SOLUBILITY: Soluble in 100% ethanol or ether; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: MAY BE MUTAGENIC. IRRITANT. MAY BE CARCINOGENIC.

Product Description
Reduces NF-κB activation through the formation of conjugates with NF-κB.
Product Specific Literature References
Acrolein causes inhibitor kappaB-independent decreases in nuclear factor kappaB activation in human lung adenocarcinoma (A549) cells: N.D. Horton, et al.; J. Biol. Chem. 274, 9200 (1999) Abstract; Full Text
 
 
ALX-840-040 Revised 09-Oct-07
DNA (purified) (DNAse I activated)
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-840-040-C010   10 µg 58.00 USD Add To Cart
Product Specification
SOURCE/HOST: Purified from calf thymus and activated by digestion with DNAse I.
CONCENTRATION: 0.2mg/ml
PURITY: ≥99% (SDS-PAGE)
FORMULATION: Liquid. In TRIS-EDTA.
APPLICATION: For use with PARP-1 (Prod. No. ALX-201-063) or PARP-2 (Prod. No. ALX-201-064) in vitro activity tests.
For details please see protocol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Images
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Product Specific Literature References
PARP-2, A novel mammalian DNA damage-dependent poly(ADP-ribose) polymerase: J.C. Ame, et al.; J. Biol. Chem. 274, 17860 (1999) Abstract; Full Text
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Further Categories Containing This Product:
PARP Other Products
 
 
ALX-201-278 Revised 30-Sep-08
DNA Ligase I (human) (recombinant) (His)
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SYNONYMS DNA Ligase 1 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Replication Other Products
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ALX-201-278-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~102kDa. Note: The mobility of DNA ligase I in a PAA gel is not corresponding to its MW; it runs at ~125kDa.
SOURCE/HOST: Produced in E. coli. Full length human DNA ligase I is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.6mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM HEPES-KOH, pH 7.5, containing 30mM sodium chloride, 1mM EDTA, 1mM DTT and 30% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
USE/STABILITY: Enzyme is freshly produced upon customer request and is stable for ~2 weeks at -80°C. Very prone to degradation even in the absence of proteases. Freeze in liquid nitrogen for long term storage.
HANDLING: After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.
Product Specific Literature References
The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair: A. Gembka, et al.; Nucleic Acids Res. 35, 2596 (2007) Abstract; Full Text
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • DNA Repair Other Products
 
 
ALX-201-295 Revised 20-Oct-08 New product
DNA Polymerase β (human) (recombinant)
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SYNONYMS Pol β (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-295-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~39kDa
EC: 2.7.7.7, 4.2.99.-
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 0.35mg/ml
PURITY: ≥98% (SDS-PAGE).
FORMULATION: Liquid. In 20mM TRIS, pH 7.5, containing 20% (v/v) glycerol, 1mM DTT and 100mM sodium chloride.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for more then 1 year when stored at -20°C or -80°C.
HANDLING: Avoid more than 5 freeze/thaw cycles.
Product Specific Literature References
Purification and domain-mapping of mammalian DNA polymerase beta: W.A. Beard & S.H. Wilson; Methods Enzymol. 262, 98 (1995) Abstract
General Information
During base excision repair (BER) DNA polymerase β (Pol β) is recruited by APE1. Pol β has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol β also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol β plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression of Pol β, as found in some human tumors, could confer an increase in spontaneous mutagenesis. It has been further suggested that Pol β does not excise 5’-oxidized AP sites induced by reactive oxygen species (ROS).
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P06746: DNA polymerase beta
General Literature References
Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation: S. Grösch, et al.; Cancer Res. 58, 4410 (1998) Abstract; Full Text
Cells deficient in DNA polymerase beta are hypersensitive to alkylating agent-induced apoptosis and chromosomal breakage: K. Ochs, et al.; Cancer Res. 59, 1544 (1999) Abstract; Full Text
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-276 Revised 05-Dec-07
DNA Polymerase λ (human) (recombinant)
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SYNONYMS Pol λ (human) (recombinant)
DNA Polymerase κ (human) (recombinant)
DNA Polymerase β2 (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-276-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~64kDa
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 2.5mg/ml
PURITY: ≥98% (SDS-PAGE).
FORMULATION: Liquid. In 40mM TRIS/HCl, pH 7.5, containing 150mM NaCl, 1mM DTT, 1mM β-mercaptoethanol and 50% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for several months when stored at -20°C or -80°C.
HANDLING: Avoid freeze/thaw cycles.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-077 Revised 10-Jul-07
DNase I-like Endonuclease 3 (human) (recombinant)
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SYNONYMS