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ALX-850-020 Revised 23-Jul-08
Annexin V-FITC Apoptosis Detection Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-020-KI02   100 tests 360.00 USD Add To Cart
ALX-850-020-KI01   300 tests 540.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Annexin V-FITC: 100 tests/500µl; 300 tests/1.5ml;
Liquid. In 50mM TRIS, pH 7.4, 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
Binding Buffer: 50ml (4x concentrated)
Concentration after dilution: 10mM HEPES/NaOH, pH 7.4, 140mM sodium chloride, 2.5mM CaCl2.
Propidium Iodide: 20µg/ml (100 tests: 1.6ml, 300 tests: 2 x 1.6ml).
PURITY: ≥98% (SDS-PAGE; HPLC)
APPLICATION: For the detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening annexin V-FITC, aliquot contents and freeze at -20°C.
Product Description
FITC-labelled recombinant human annexin V shows bright green fluorescence (Ex(max): 488nm, Em(max): 530nm).
Further Categories Containing This Product:
Detection Kits
 
 
ALX-850-250 Revised 22-Apr-08
Annexin V-FITC Apoptosis Detection Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-250-KI01   25 tests 175.00 USD Add To Cart
ALX-850-250-KI02   100 tests 357.00 USD Add To Cart
ALX-850-250-KI03   400 tests 875.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Annexin V-FITC, propidium iodide solution and binding buffer.
An assay protocol is included.
APPLICATION: For the detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.
Product Description
FITC-labelled recombinant human annexin V shows shows bright green fluorescence (Ex(max): 488nm; Em(max): 530nm).
General Information
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Detection Kits
 
 
ALX-850-256 Revised 24-Feb-05
Annexin V-PE Apoptosis Detection Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-256-KI01   25 tests 189.00 USD Add To Cart
ALX-850-256-KI02   100 tests 371.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Kit contains annexin V-PE and binding buffer. An assay protocol is included.
APPLICATION: For the detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Further Categories Containing This Product:
Detection Kits
 
 
ALX-850-049 Revised 17-Apr-08
Annexin V (human) ELISA Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-850-049-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 0.33ng/ml (range 0.8 to 50ng/ml)
QUANTITY: 96wells (~80tests).
APPLICATION: For the quantitative detemination of human annexin V in cell culture supernatant and serum.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-850-040 Revised 17-Apr-08
anti-Annexin V (human) ELISA Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-850-040-KI01   1 Kit 619.00 USD Add To Cart
Product Specification
SENSITIVITY: 1.18ng/ml (range 6.25 to 400ng/ml)
QUANTITY: 96 wells (~80 tests).
APPLICATION: For the quantitative determination of human autoantibodies to annexin V (Vac-α) in cell culture supernatants, serum and other body fluids.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
Lupus anticoagulant autoantibody induces apoptosis in umbilical vein endothelial cells: involvement of annexin V: N. Nakamura, et al.; BBRC 205, 1488 (1994) Abstract
Thrombosis in patients with SLE and antiphospholipid syndrome: J. Kaburaki, et al.; Rinsho Ketsueki 36, 320 (1995), (Article in Japanese) Abstract
Annexin V autoantibodies in rheumatoid arthritis: M.I. Rodriguez-Garcia, et al.; Ann. Rheum. Dis. 55, 895 (1996) Abstract
Clinical significance of anti-annexin V antibodies in patients with systemic lupus erythematosus: J. Kaburaki, et al.; Am. J. Hematol. 54, 209 (1997) Abstract
General Information
MANUFACTURER Manufactured by Bender MedSystems.
Further Categories Containing This Product:
ELISA & EIA Kits
 
 
ALX-850-255 Revised 12-Aug-08
Annexin V-FITC Apoptosis Detection Kit Plus
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Annexin V / Related Products
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ALX-850-255-KI01   25 tests 203.00 USD Add To Cart
ALX-850-255-KI02   100 tests 413.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Annexin V-FITC, SYTOX green dye and binding buffer.
An assay protocol is included.
APPLICATION: For the detection of apoptotic cells by flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.
Product Description
FITC-labelled recombinant human annexin V shows bright green fluorescence. SYTOX green dye is impermeant to live cells and apoptotic cells, but stains necrotic cells with intense green fluorescence (Ex(max): 488nm; Em(max): 530nm). Apoptotic cells show green fluorescence, dead cells show a higher level of green fluorescence and live cells show little or no fluorescence. By using the FL1 channel in FC, other channels are free for multiple labelling experiments.
General Information
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Detection Kits
 
 
ALX-850-318 Revised 14-Feb-07
Antioxidant Assay Kit
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PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Oxidative Stress Markers & Reagents / Related Products
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ALX-850-318-KI01   1 Kit 349.00 USD Add To Cart
Product Specification
QUANTITY: 96 wells (~80 tests)
APPLICATION: For the rapid measurement of antioxidant capacity in a variety of sample types, including plasma, serum, urine, saliva, and cell lysates.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Description
This antioxidant assay is designed to measure the overall antioxidant capacity within a given sample. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS in comparison to trolox, a water-soluble tocopherol analog. By quantifying the cumulative effect of all antioxidants present, more relevant biological information is acquired compared with the measurement of individual components alone. The assay relies on the ability of antioxidants in the sample to inhibit the oxidation of ABTS® (2,2’-azino-di-[3-ethylbenzthiazoline sulphonate]) to ABTS®·+ by metmyoglobin.
Further Categories Containing This Product:
Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Quantification Kits
 
 
ALX-850-042 Revised 12-Jul-05
APO-BRDU™ Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY BrdU
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-042-KI01   1 Kit 540.00 USD Add To Cart
Product Specification
QUANTITY: 50 assays.
KIT/SET CONTAINS: Contains all the reagents necessary, including positive and negative control cells.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -20°C
POSITIVE CONTROL INCLUDED: yes
Product Description
Kit for measuring apoptosis with high sensitivity by Dual Color Flow Cytometry in mammalian cells. The kit differs from the APO-DIRECT™ Kit (Prod. No. ALX-850-041) by labelling the 3'-hydroxyl termini of apoptotic DNA ends with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). Br-dUTP is more readily incorporated into the genome of apoptotic cells than are larger ligands like fluorescein, biotin, etc. This greater incorporation coupled with the use of a fluorescein labelled anti-BrdU monoclonal antibody (Prod. No. ALX-804-197) gives  rise to a stronger signal in flow cytometry.
Product Specific Literature References
Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry 13, 795 (1992) Abstract
Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation: X. Li & Z. Darzynkiewicz; Cell Prolif. 28, 571 (1995) Abstract
Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color: X. Li, et al.; Exp. Cell. Res. 222, 28 (1996) Abstract
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry 27, 1 (1997), (Review) Abstract
General Information
MANUFACTURER Manufactured by Phoenix Flow Systems.
Further Categories Containing This Product:
Detection Kits
 
 
ALX-850-041 Revised 29-Jan-05
APO-DIRECT™ Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY DNA-based Apoptosis Detection
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-041-KI01   1 Kit 540.00 USD Add To Cart
Product Specification
QUANTITY: 50 assays.
KIT/SET CONTAINS: Contains all the reagents necessary, including positive and negative control cells.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: +4°C
POSITIVE CONTROL INCLUDED: yes
Product Description
One-step flow cytometry kit for the qualitative and quantitative measurement of apoptosis in mammalian cells. One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. The large number of DNA fragments appearing in apoptotic cells results in a multitude of 3'-hydroxyl termini of DNA ends which can be identified with fluorescent tagged deoxyuridine triphosphate nucleotides (F-dUTP). The enzyme terminal deoxynucleotidyl transferase (TdT) catalyzes a template independent addition of deoxyribonucleoside triphosphates (e.g. F-dUTP) to the 3'h-hydroxyl ends of double- or single-stranded DNA. A substantial number of these sites are available in apoptotic cells providing the basis for the single step fluorescent labelling and flow cytometric method utilized in the Apo-DIRECT™ Kit.
Product Specific Literature References
Features of apoptotic cells measured by flow cytometry: Z. Darzynkiewicz, et al.; Cytometry 13, 795 (1992) Abstract
Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis): Z. Darzynkiewicz, et al.; Cytometry 27, 1 (1997), (Review) Abstract
Single-step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides: detection of apoptosis and bromodeoxyuridine incorporation: X. Li, et al.; Cytometry 20, 172 (1995) Abstract
General Information
MANUFACTURER Manufactured by Phoenix Flow Systems.
Further Categories Containing This Product:
Detection Kits
 
 
ALX-850-248 Revised 22-Apr-08
ApoSENSOR™ ADP/ATP Ratio Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apoptosis & Necrosis Detection Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-248-KI01   200 tests 455.00 USD Add To Cart
Product Specification
QUANTITY: 200 assays.
KIT/SET CONTAINS: 50ml Nucleotide Releasing Buffer, 1 vial ATP Monitoring Enzyme, 1 vial ADP Converting Enzyme, 500µl Enzyme Reconstitution Buffer.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Protect from light.
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Product Description
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
The assay utilizes bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light fom ATP and luciferin. The light can be measured using a luminometer or beta counter. ADP levels are measured by its conversion to ATP that is subsequently detected using the same reaction.
This highly sensitive assay (detects 100 mammalian cells/well) can be fully automated for high throughput measurements. The ADP/ATP ratio assay offers highly consistent results, with excellent correlation to other apoptosis markers (e. g. TUNEL-based techniques and caspase assays).
General Information
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Quantification Kits
 
 
ALX-850-247 Revised 22-Apr-08
ApoSENSOR™ Cell Viability Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apoptosis & Necrosis Detection Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-247-KI01   200 tests 203.00 USD Add To Cart
ALX-850-247-KI02   1000 tests 609.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Nucleotide Releasing Buffer, ATP Monitoring Enzyme, Enzyme Reconstitution Buffer, ATP.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
Product Images
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Product Description
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
General Information
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Quantification Kits
 
 
APO-54N-020 Revised 02-Apr-08
BAFF, Soluble (human) ELISA Kit
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SYNONYMS BlyS, Soluble (human) ELISA Kit
TALL-1, Soluble (human) ELISA Kit
THANK, Soluble (human) ELISA Kit
zTNF4, Soluble (human) ELISA Kit
TNFSF 13B/20, Soluble (human) ELISA Kit
CD257, Soluble (human) ELISA Kit
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY BAFF
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APO-54N-020-KI01   1 Kit 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human