Activity Assay:
1) Serially dilute Vitronectin 10-fold with PBS. Place 100µl of each dilution in a microtiter plate, cover and incubate at 37°C for 2 hours.
2) After washing wells with PBS, add 200µl of 1% BSA-PBS solution and incubate at 37°C for 1 hour.
3) After washing wells with PBS, incubate the coated wells with NRK-49F cells suspended in SF-DMEM (Serum Free-Dulbecco’s Modified Eagle Medium) at 37°C for 4 hours under 5% CO₂.
4) Check cells by microscope.

Swiss-Prot link P04004: Vitronectin (human) (precursor)
AfCS Signalling Gateway link 002367: Vitronectin (mouse)