ALEXIS Biochemicals: cAMP-dependent Protein Kinase (Holoenzyme Type Iα, Cα2 (mouse)/RIα2 (bovine)) (recombinant)

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ALX-201-228

Revised 07-Sep-05

cAMP-dependent Protein Kinase (Holoenzyme Type Iα, Cα2 (mouse)/RIα2 (bovine)) (recombinant)
SYNONYMS	Protein Kinase A (Holoenzyme Type Iα, Cα₂(mouse)/RIα₂ (bovine)) (recombinant) PKA (Holoenzyme Type Iα, Cα₂(mouse)/RIα₂ (bovine)) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PKA

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-228-C020		20 µg	474.00 USD
ALX-201-228-C050		50 µg	812.00 USD
ALX-201-228-C100		100 µg	1'489.00 USD

Product Specification

MW:	~168kDa
SOURCE/HOST:	Produced in E. coli. Inactive holoenzyme consisting of one dimeric bovine regulatory subunit type Iα and two monomeric mouse catalytic subunits. Can be activated by adding the second messenger cAMP (Prod. No. ALX-480-011) releasing two monomeric catalytic subunits.
FORMULATION:	Liquid. In 20mM MOPS, pH 7.0, containing 5mM MgCl₂, 100µM ATP, 150mM NaCl and 50% glycerol.
SPECIFIC ACTIVITY:	1.5 x 10⁷U/mg (for the Cα catalytic subunit of the activated holoenzyme). One unit is defined as the amount of enzyme required to incorporate 1pmol phosphate per min into the specific substrate kemptide (Prod. No. ALX-160-006) at 30°C.
APPLICATION:	Suitable for analyzing PKA type I agonists (cAMP analogs) or antagonists.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	In order to prevent dissociation of the holoenzyme complex use always buffer with 5-10mM MgCl₂ and 0.1-1mM ATP.
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

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