ALEXIS Biochemicals: cAMP-dependent Protein Kinase (Regulatory Subunit RIIβ) (rat) (recombinant)

ALX-201-227

Revised 14-Nov-07

cAMP-dependent Protein Kinase (Regulatory Subunit RIIβ) (rat) (recombinant)
SYNONYMS	Protein Kinase A (Regulatory Subunit RIIβ) (rat) (recombinant) PKA (Regulatory Subunit RIIβ) (rat) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PKA

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-227-C025		25 µg	474.00 USD
ALX-201-227-C050		50 µg	812.00 USD
ALX-201-227-C100		100 µg	1'489.00 USD

Product Specification

MW:	45kDa/subunit; homodimer
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 10mM potassium phosphate, pH 6.8, containing 150mM NaCl, 14mM 2-mercaptoethanol, 1mM EDTA and 50% glycerol.
BIOLOGICAL ACTIVITY:	Reversibly inhibits the catalytic subunit Cα of cAMP-dependent protein kinase (PKA) (Prod. No. ALX-201-224). The inhibition of the catalytic subunit can be reversed by the addition of the second messenger cAMP (>1µM) (Prod. No. ALX-480-011).
APPLICATION:	Suitable for inhibition studies and for the analysis of interactions with so-called A-kinase anchoring proteins (AKAPs).
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Keep the glycerol stock solution of the enzyme permanently at -20°C while pipetting to avoid loss of inhibitory activity.
HANDLING:	Avoid freeze/thaw cycles.

General Literature References

cAMP signaling in neurons: patterns of neuronal expression and intracellular localization for a novel protein, AKAP 150, that anchors the regulatory subunit of cAMP-dependent protein kinase II beta: S.B. Glantz, et al.; Mol. Biol. Cell 3, 1215 (1992) Abstract
Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of cAMP-dependent protein kinase II beta: A.H. Hirsch, et al.; J. Biol. Chem. 267, 2131 (1992) Abstract
Regional localization of the regulatory subunit (RII beta) of the type II cAMP-dependent protein kinase in human brain: V. Licameli, et al.; Brain. Res. 578, 61 (1992) Abstract
Identification of a high affinity binding protein for the regulatory subunit RII beta of cAMP-dependent protein kinase in Golgi enriched membranes of human lymphoblasts: R.M. Rios, et al.; EMBO J. 11, 1723 (1992) Abstract
Characterization of distinct tethering and intracellular targeting domains in AKAP75, a protein that links cAMP-dependent protein kinase II beta to the cytoskeleton: S.B. Glantz, et al.; J. Biol. Chem. 268, 12796 (1993) Abstract
Phosphorylation of the regulatory subunit of type II beta cAMP-dependent protein kinase by cyclin B/p34cdc2 kinase impairs its binding to microtubule-associated protein 2: G. Keryer, et al.; PNAS 90, 5418 (1993) Abstract
Mutagenesis of the regulatory subunit (RII beta) of cAMP-dependent protein kinase II beta reveals hydrophobic amino acids that are essential for RII beta dimerization and/or anchoring RII beta to the cytoskeleton: Y. Li & C.S. Rubin; J. Biol. Chem. 270, 1935 (1995) Abstract
Overexpression of RII beta regulatory subunit of protein kinase A in human colon carcinoma cell induces growth arrest and phenotypic changes that are abolished by site-directed mutation of RII beta: M. Nesterova, et al.; Eur. J. Biochem. 235, 486 (1996) Abstract
Defective motor behavior and neural gene expression in RIIbeta-protein kinase A mutant mice: E.P. Brandon, et al.; J. Neurosci. 18, 3639 (1998) Abstract

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

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