ALEXIS Biochemicals: cGMP-dependent Protein Kinase Iβ (human) (recombinant)

ALX-201-071

Revised 12-Jul-06

cGMP-dependent Protein Kinase Iβ (human) (recombinant)
SYNONYMS	Protein Kinase G Iβ (human) (recombinant) cGK Iβ (bovine) (recombinant) PKG Iβ (human) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PKG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-071-C005		5 µg	320.00 USD

Product Specification

MW:	~76kDa; monomer.
EC:	2.7.1.37
SOURCE/HOST:	Produced in Sf9 cells [1].
CONCENTRATION:	50µg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS, pH 7.4, containing 1mM EDTA, 1mM β-mercaptoethanol, 100mM NaCl, 10U/ml trasylol and 50% glycerol.
SPECIFIC ACTIVITY:	~1.5-5U/mg protein. 25-fold stimulation by cGMP (5µM). One unit corresponds to 1µmol/mg/min using VASPtide (RRKVSKQE) as substrate under the following optimal conditions: 10mM HEPES, pH 7.4, containing 5mM MgCl₂, 1mM DTE, 0.2mM EDTA.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Enzyme is relatively unstable. To prevent loss of activity when performing experiments, keep the stock vial of the enzyme on ice at all times. Enzyme loses activity during a single freeze/thaw cycle.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Specific Literature References

[1] Expression, purification, and characterization of the cGMP-dependent protein kinases I beta and II using the baculovirus system: D. Pöhler, et al.; FEBS Lett. 374, 419 (1995) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P14619: PKG Iβ (human)
AfCS Signalling Gateway link A000043: PKG I (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins / Fusion Proteins

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