ALEXIS Biochemicals: cGMP-dependent Protein Kinase II (rat) (recombinant)

ALX-201-072

Revised 06-Oct-06

cGMP-dependent Protein Kinase II (rat) (recombinant)
SYNONYMS	PKG II (rat) (recombinant) cGK II (rat) (recombinant) Protein Kinase G II (rat) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	PKG

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-072-C005		5 µg	320.00 USD

Product Specification

MW:	~86kDa; monomer.
EC:	2.7.1.37
SOURCE/HOST:	Produced in Sf9 cells [1].
CONCENTRATION:	50µg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS, pH 7.4, containing 1mM EDTA, 5mM β-mercaptoethanol, 100mM NaCl, 10U/ml trasylol, 0.015% NP40 and 50% glycerol.
SPECIFIC ACTIVITY:	~0.5-2.0U/mg protein. 5-fold activation by cGMP (5µM). One unit corresponds to 1µmol/mg/min using VASPtide (RRKVSKQE) as substrate, under the following optimal conditions: 10mM HEPES (pH 7.4), 5mM MgCl₂, 1mM DTE, 0.2mM EDTA.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	The enzyme is relatively unstable. To prevent loss of activity when performing experiments, keep the stock vial of the enzyme on ice (0-4°C) at all times. The enzyme loses activity during a single freeze/thaw cycle. To avoid adherence of the product to plastic, the use of siliconized vials and tips is recommended.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Specific Literature References

[1] Expression, purification, and characterization of the cGMP-dependent protein kinases I beta and II using the baculovirus system: D. Pöhler, et al.; FEBS Lett. 374, 419 (1995) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q64595: cGMP-dependent Kinase II (rat)
AfCS Signalling Gateway link A000044: cGMP-dependent Kinase II (mouse)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Enzymes

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