ALEXIS Biochemicals: iODN 2088 (TLRgrade™) (synthetic) (Inhibitory for mouse)

ALX-746-250

Revised 16-Sep-08

iODN 2088 (TLRgrade™) (synthetic) (Inhibitory for mouse)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Oligodeoxynucleotides [ODNs]

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-746-250-T050		50 tests	155.00 USD

Product Specification

SEQUENCE:	5’-tcctggcggggaagt-3’ (lower case letters indicate phosphorothioate linkage).
MW:	4887 (ammonium salt)
SOURCE/HOST:	Synthetic.
QUANTITY:	40nmol (~200µg). Sufficient for ~50 cellular inhibition assays. Working concentration depends upon concentration, type and MW of stimulating mouse CpG ODN, the cellular system and read-out used. Titrate at 3-10-fold molar excess for inhibition.
PURITY DETAIL:	Endotoxin tested - TLRgrade™.
FORMULATION:	Lyophilized. Sterile.
ENDOTOXIN CONTENT:	<0.002EU/µg (LAL test; BioWhittaker)
RECONSTITUTION:	For a 100µM stock solution, dissolve the total vial content in 400µl endotoxin-free water (included) or PBS (to order separately). To obtain optimal dissolving we recommend the following procedure: - Add 50% of the solvent and let dissolve for 10 min. - Add remaining 50% of the solvent and mix thoroughly. - Moderate warming may aid dissolving.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Aqueous stock solution is stable for 1 day when stored at +4°C.
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. Protect from light. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Description

Inhibitory iODN oligodeoxynucleotide (mouse) with phosphorothioate backbone. For active mouse CpG compounds see e.g. ODN 1668 (Prod. No. ALX-746-001).

General Information

BACKGROUND/TECHNICAL INFORMATION

Includes 1 vial of ddWater (endotoxin-free) (Prod. No. ALX-505-008).

Related Products:

- PBS (endotoxin-free) (Prod. No. ALX-505-007)

- ODN 1668 (TLRgrade™) (synthetic) (Prod. No. ALX-746-001)
- ODN 1826 (TLRgrade™) (synthetic) (Prod. No. ALX-746-002)
- ODN 1585 (TLRgrade™) (synthetic) (Prod. No. ALX-746-003)
- ODN 2006 (TLRgrade™) (synthetic) (Prod. No. ALX-746-006)
- ODN 2395 (TLRgrade™) (synthetic) (Prod. No. ALX-746-020)

General Literature References

Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation: I. Gursel, et al.; J. Immunol. 171, 1393 (2003) Abstract
Suppressive oligodeoxynucleotides inhibit Th1 differentiation by blocking IFN-gamma- and IL-12-mediated signaling: H. Shirota, et al.; J. Immunol. 173, 5002 (2004) Abstract
Inhibitors of TLR-9 act on multiple cell subsets in mouse and man in vitro and prevent death in vivo from systemic inflammation: O. Duramad, et al.; J. Immunol. 174, 5193 (2005) Abstract
Suppressive oligodeoxynucleotides protect mice from lethal endotoxic shock: H. Shirota, et al.; J. Immunol. 174, 4579 (2005) Abstract
Therapeutic potential of oligonucleotides expressing immunosuppressive TTAGGG motifs: D.M. Klinman, et al.; Ann. NY Acad. Sci. 1058, 87 (2005) Abstract

ALX-746-006	ODN 2006 (TLRgrade™) (synthetic)
ALX-746-003	ODN 1585 (TLRgrade™) (synthetic)
ALX-746-001	ODN 1668 (TLRgrade™) (synthetic)
ALX-746-002	ODN 1826 (TLRgrade™) (synthetic)
ALX-505-007	PBS (endotoxin-free)
ALX-505-008	ddWater (endotoxin-free)
ALX-746-020	ODN 2395 (TLRgrade™) (synthetic)