ALEXIS Biochemicals: p34cdc2/Cyclin B (starfish Marthasterias glacialis) (purified)

ALX-202-036

Revised 04-Mar-05

p34cdc2/Cyclin B (starfish Marthasterias glacialis) (purified)
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	CDKs and Cyclins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-202-036-R050		50 µl	360.00 USD

Product Specification

SOURCE/HOST:	Isolated from starfish oocytes (Marthasterias glacialis).
PURITY:	Affinity-purified
FORMULATION:	Liquid. 50µl in 200mM sodium bicarbonate (pH 8.2), 0.2M sodium chloride and 10% glycerol.
SPECIFIC ACTIVITY:	~6,000U/ml. One unit is defined as the amount of enzyme that catalyzes the transfer of 1.0pmol of phosphate to histone H1 per minute at 30°C, pH 7.2. Recommended buffer for assay: 60mM β-glycerophosphate, 15mM p-nitrophenylphosphate, 25mM MOPS (pH 7.2), 5mM EGTA, 15mM MgCl₂, 1mM DTT, 1mM sodium vanadate. Use 1mg histone H1/ml.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

Product Specific Literature References

MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B: J.C. Labbé; EMBO J. 8, 3053 (1989) Abstract
Cyclin B targets p34cdc2 for tyrosine phosphorylation: L. Meijer, et al.; EMBO J. 10, 1545 (1991) Abstract
Interaction between the cell-cycle-control proteins p34cdc2 and p9CKShs2. Evidence for two cooperative binding domains in p9CKShs2: L. Azzi, et al.; Eur. J. Biochem. 203, 353 (1992) Abstract
Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase: B. Baratte, et al.; Anticancer Res. 12, 873 (1992) Abstract
Inhibition of cyclin-dependent kinases by purine analogues: J. Veselý, et al.; Eur. J. Biochem. 224, 771 (1994) Abstract

General Information

p34^cdc2/cyclin B is a serine/threonine protein kinase. Consists of the catalytic cdc2 subunit (MW 32kDa) and the activating regulatory subunit cyclin B (MW 47kDa). Major regulator of the eukaryotic cell cycle, at the G2/M transition level.

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